All recombinant enzymes are purified chromatographically
to achieve a high percentage purity. Enzymes are typically
greater than 90% purity. The recombinant clones used to
make the enzymes have been DNA sequence to confirm identity
to the wild type enzyme (ensuring that the recombinant enzymes
have no mutations). We do not assay enzymes for contaminating
enzymes. Therefore, the customer should test the suitability
of an enzyme for a particular application on a small scale
prior to purchasing a bulk quantity of a lot. The enzymes
produced by Bayou Biolabs are provided to customer "as
is" without any warranty of merchantability or fitness
for a particular purpose and without any other warranty,
express or implied..
Supplied:Enzymes are supplied in glycerol buffer. Enzymes
are shipped on dry ice at customer’s expense. Please
see the table below for enzymes currently available.
Bulk quantities are available at low cost. Standard bulk
quantities are 100 mg, 500 mg, 1 gram, 5 grams, and 10
grams. We can also package any bulk quantity specified
by customer. We have capability for scaling up to produce
batches of several hundred grams. Please contact customer
service for price and availability.
Custom production: We
can custom produce milligram to multiple gram quantities
of any protein or enzyme. In general, we use high fidelity
PCR to amplify the gene of interest and clone the gene
in our proprietary expression vector. We recommend using
polyhistidine tags to facilitate enzyme purification,
although we can also produce native and GST tagged proteins.
Several recombinant clones are selected for DNA sequencing
to obtain a clone with no mutations. Fermentation of the
desired clone is performed at the desired scale (8 liters,
16 liters, 100 liters, 400 liters, 800 liters), and the
protein is purified chromatographically according to the
customer’s specifications, in order to obtain desired
purity. Production can be customized to accommodate customer
needs. Please contact customer service for a price quote.