Here
is an example of our amazing supercoiled purification option.
We are the world's only provider of this service - for any
scale, and at low cost.
Gel
1: ethidium bromide stained gel of an M13mp18 double
stranded plasmid preparation - before and after supercoiled
purification
Left lane: plasmid preparation
before supercoiled purification. The lower band is the supercoiled
plasmid. The upper band is nicked plasmid.
Right lane: plasmid preparation
after supercoiled purification. Note that all the nicked plasmid
has been removed.
Gel 2: ethidium bromide stained
gel of a 10 kilobase plasmid preparation - before and after
supercoiled purification.
Left lane: plasmid preparation
before supercoiled purification. The lower band is the supercoiled
plasmid. The upper band is nicked plasmid.
Right lane: plasmid preparation
after supercoiled purification. Note that all the nicked plasmid
has been removed. The remaining upper band is dimeric supercoiled
plasmid.
