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Applications: We produce high quality, low cost plasmid for animal and in vitro applications. We do NOT produce plasmid for human use. We do NOT produce plasmid for generating virus for human use (e.g. adenovirus, retrovirus, and any other viruses).

Experience: We have offered the custom plasmid preparation service for since 1996. We have experience in making bulk quantities of all types and sizes of plasmids. We routinely supply bulk plasmid to customers in the pharmaceutical and gene therapy industries. Our service is completely confidential. You only supply us with a small sample (1 ug) of your plasmid or the plasmid in an E. coli host. We do the rest.

Four Scales: We offer five scales - 8 liters, 16 liters, 32 liters, 100 liters, and 400 liters. For the 8, 16, and 32 liter scales, the bacterial host is grown in shaker flasks. For the 100 and 400 liter scales, the bacterial host is grown in industrial fermentors. The cost per liter for the service falls dramatically for larger scales.

Yield: Our plasmid yield is usually double the yield of a preparation grown in LB broth. We use a proprietary rich broth formulation to achieve this higher yield. Typical yields for pUC based plasmids are between 10 to 15 mg per liter. We routinely achieve even higher yields. Our record yield is 32 mg per liter. Our average yields for 8 and 16 liter scales are 100 mg and 200 mg respectively. The yield for 100 liter scale ranges from 1 - 2 grams. Yield is highly dependent on the individual plasmid. At the request of the customer, we can obtain an estimated plasmid yield prior to large scale fermentation. If the estimated yield for a plasmid is unacceptably low for the customer, the customer may cancel the preparation at no cost.

Plasmid Quality: The plasmid is prepared by our own proprietary plasmid preparation process. The bioactivity of our plasmid is similar to the bioactivity of Qiagen plasmid. Plasmid is typically greater than 90% supercoiled. The final plasmid product is low in endotoxin, RNA, protein, RNase, DNase, nucleotide, and nucleoside contamination. Plasmid is not exposed to UV radiation or to mutagenic ethidium bromide, ensuring maximal biological activity.

Supercoiled Purification: We are the only company in the world to offer the highest purity possible - supercoiled purified plasmid. In the supercoiled purification, we remove most of the nicked plasmid and most of the residual chromosomal DNA contamination. We can perform this process on any scale - from 8 liters to 400 liters and higher. The supercoiled purification process is highly efficient. Less than 5% of the supercoiled plasmid is lost in the process. The final plasmid product is usually greater than 99% supercoiled. This process is proprietary. The supercoiled purification is optional. Click Here for Examples of supercoiled purification shown in the gel photos.

Buffer Selection: The final plasmid product is usually supplied as a dry pellet. Alternatively, the plasmid can be supplied in any buffer and at any concentration requested by the customer.

Quality Control: The final plasmid product is checked by agarose gel electrophoresis to confirm plasmid size and purity. The plasmid product is supplied with a data sheet which includes the total yield, plasmid concentration, Abs260/Abs280 ratio, and a gel photograph.

Fast Service: One week turn around time for the 8 and 16 liter scale. Two week turn around time for the 100 liter scale. The final plasmid product is shipped overnight delivery at the customer's expense - usually about $30 total.

Warranty: The plasmid materials produced by Bayou Biolabs are experimental in nature and are provided to customer "as is" without any warranty of merchantability or fitness for a particular purpose and without any other warranty, express or implied.

Discounts are available for multiple plasmid preparations.

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