We produce high quality, low cost plasmid for animal and
in vitro applications. We do NOT produce plasmid for human
use. We do NOT produce plasmid for generating virus for
human use (e.g. adenovirus, retrovirus, and any other viruses).
We have offered the custom plasmid preparation service for
since 1996. We have experience in making bulk quantities
of all types and sizes of plasmids. We routinely supply
bulk plasmid to customers in the pharmaceutical and gene
therapy industries. Our service is completely confidential.
You only supply us with a small sample (1 ug) of your plasmid
or the plasmid in an E. coli host. We do the rest.
We offer five scales - 8 liters, 16 liters, 32 liters,
100 liters, and 400 liters. For the 8, 16, and 32 liter
scales, the bacterial host is grown in shaker flasks.
For the 100 and 400 liter scales, the bacterial host is
grown in industrial fermentors. The cost per liter for
the service falls dramatically for larger scales.
Yield: Our plasmid yield
is usually double the yield of a preparation grown in
LB broth. We use a proprietary rich broth formulation
to achieve this higher yield. Typical yields for pUC based
plasmids are between 10 to 15 mg per liter. We routinely
achieve even higher yields. Our record yield is 32 mg
per liter. Our average yields for 8 and 16 liter scales
are 100 mg and 200 mg respectively. The yield for 100
liter scale ranges from 1 - 2 grams. Yield is highly dependent
on the individual plasmid. At the request of the customer,
we can obtain an estimated plasmid yield prior to large
scale fermentation. If the estimated yield for a plasmid
is unacceptably low for the customer, the customer may
cancel the preparation at no cost.
The plasmid is prepared by our own proprietary plasmid
preparation process. The bioactivity of our plasmid is
similar to the bioactivity of Qiagen plasmid. Plasmid
is typically greater than 90% supercoiled. The final plasmid
product is low in endotoxin, RNA, protein, RNase, DNase,
nucleotide, and nucleoside contamination. Plasmid is not
exposed to UV radiation or to mutagenic ethidium bromide,
ensuring maximal biological activity.
We are the only company in the world to offer the highest
purity possible - supercoiled purified plasmid. In the
supercoiled purification, we remove most of the nicked
plasmid and most of the residual chromosomal DNA contamination.
We can perform this process on any scale - from 8 liters
to 400 liters and higher. The supercoiled purification
process is highly efficient. Less than 5% of the supercoiled
plasmid is lost in the process. The final plasmid product
is usually greater than 99% supercoiled. This process
is proprietary. The supercoiled purification is optional.
for Examples of supercoiled purification shown in the
The final plasmid product is usually supplied as a dry
pellet. Alternatively, the plasmid can be supplied in
any buffer and at any concentration requested by the customer.
The final plasmid product is checked by agarose gel electrophoresis
to confirm plasmid size and purity. The plasmid product
is supplied with a data sheet which includes the total
yield, plasmid concentration, Abs260/Abs280 ratio, and
a gel photograph.
One week turn around time for the 8 and 16 liter scale.
Two week turn around time for the 100 liter scale. The
final plasmid product is shipped overnight delivery at
the customer's expense - usually about $30 total.
Warranty: The plasmid
materials produced by Bayou Biolabs are experimental in
nature and are provided to customer "as is"
without any warranty of merchantability or fitness for
a particular purpose and without any other warranty, express
Discounts are available for multiple
8 liter scale (add $500 for supercoiled
16 liter scale (add $1,000 for supercoiled
32 liter scale (add $1,500 for supercoiled purification)
100 liter scale (add $2,500 for supercoiled