Ready to Ligate
pUC18-SmaI-AP and M13mp18-SmaI-AP
50 cents per ligation
$150 for 10 ug. Sufficient for 200 ligations (using 50 ng
per ligation) or 100 ligations (using 100 ng per ligation).
This translates to $0.75 - $1.50 per ligation.
Highly Purified: Prepared
from pUC18 or M13mp18-SmaI by restriction digestion, alkaline
phosphatase dephosphorylation, purification by agarose gel
electrophoresis, and further purification to remove all
residual agarose. Our competitors purify the linear plasmid
by gel filtration chromatography, which does not remove
residual supercoiled and nicked plasmid, resulting in high
backgrounds. Our product is purified by agarose gel electrophoresis,
which does remove all residual supercoiled and nicked plasmid,
resulting in low backgrounds.
Ready immediately for ligation:
The dephosphorylated vectors can be ligated to any phosphorylated
DNA for cloning.
Rigorously Quality Controlled
to ensure low background transformation and intact restriction
Excellent starting material
for making double restriction digested vector for directional
cloning. An easy two hour protocol is included for making
double restriction digested plasmid from Ready to Ligate
Supplied in 100 ul TE at 100
ng/ul, a convenient concentration for routine use.
Free Shipping anywhere in
the USA by first class air mail - ships at ambient temperature.