A Breakthrough in Plasmid Purification
The Exonuclease-It™ plasmid purification system is
designed to selectively digest linear chromosomal DNA contamination
in a plasmid preparation, without significantly degrading
any supercoiled plasmid (or any covalently closed circular
plasmid). Exonuclease-It™ will also degrade open circular
plasmid and circular single stranded DNA. The Exonuclease-It™
enzyme reagent contains a proprietary mixture of exonucleases
to accomplish this task. In a typical 6 hour incubation,
Exonuclease-It™ will reduce the amount of chromosomal
DNA contamination in a plasmid preparation by 98 percent.
The by-products of the exonuclease digestion are deoxynucleoside
monophosphates. The Exonuclease-It™ plasmid purification
system is ideally used as an added step in any plasmid purification
method, in order to thoroughly remove chromosomal DNA. This
kit contains sufficient reagents for purifying 150 mg of
plasmid. Exonuclease-It™ is not for use in purifying
plasmid for human therapy.
The primary application is for removal of chromosomal DNA
contamination from plasmid preparations. However, Exonuclease-It™
may be used in any application to selectively digest linear
DNA without significantly degrading covalently closed circular
double stranded DNA. For example, a mixture of linear DNA
and covalently closed circular DNA may be generated by a
ligation reaction. Exonuclease-It™ can selectively
digest the linear DNA vector prior to transforming cells,
in order to reduce background transformants. Exonuclease-It™
may be used after Supercoil-It™. The Supercoil-It™
incubation converts open circular plasmid to supercoiled
form, and the subsequent Exonuclease-It™ incubation
removes chromosomal DNA contamination.
is supplied with sufficient 40X buffer.
Purifies 150 mg of plasmid for only $200.